Supplementary Figure 15 : Characteristics of REST+ cCREs

Supplementary Figure 15 | Characteristics of REST+ cCREs

a, Barplots depicting the number of REST+ cCREs stratified by cCRE class. b, Barplots depicting the enrichment for cCRE classes of REST+ cCREs compared to the entire Registry. c, Barplots depicting the percentage of VISTA regions with enhancer activity in a transgenic mouse enhancer assay. Regions are stratified by cCRE class and overlap with REST ChIP-seq peaks (REST+). p-values are shown for two-sided Fisher’s exact tests. d, Density plots showing the distribution of K562 STARR scores for each class of cCRE stratified by REST binding (REST+). Vertical lines denote medians which are also displayed to the right of each distribution. p-values correspond to Wilcoxon tests. e, Nested violin-boxplots showing gene expression in K562 cells for the nearest gene of different classes and categories of cCREs. On the left are cCREs annotated in K562 based on K562-specific chromatin signatures including promoter, proximal enhancer, distal enhancer and low chromatin accessbility cCREs. In the middle are REST+ cCREs stratified by cell type agnostic classification. On the right are the final REST+ cCRE categories: enhancer/silencer and silencer. Medians are shown above each distribution.